Liposome cleavage by FphH and reference enzyme PLA2
Supervisor: Dr. Fellner (Biochemistry) & Dr. Tan (Chemistry)
The Fph liposome projects are in collaboration with the Tan Lab – University of Otago – Department of Chemistry. Co-supervision between the Fellner Biochemistry and Tan Chemistry Department will strengthen these projects.
Summary: This project is ideal if you want to bridge the fields of Chemistry and Biochemistry for enzyme studies. We have established that Fphs can cleave liposomes, which mimic the bacterial membrane. Only for FphH this characterisation has been done in details and a student project would focus to perform this characterisation with additional Fph proteins and go into the chemical details of how the liposome is cleaved using various techniques, including electron microscopy.
Dye released from liposomes due to cleavage by FphA, FphB, FphD, FphE, FphF, FphH, FphI, FphJ, SAL1 and SAL2 in comparison to PLA2 and just buffer
