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Bioinks for 3D Bioprinting

Bioinks for 3D bioprinting


3D bioprinting technologies offers unprecedented automation and control over spatial localisation of multiple cells within matrices to engineer functional tissues or organs. The cells are often delivered via hydrogel-based bioinks, which are compatible with a range of 3D bioprinting modalities. In our group, we have developed a range of synthetic and natural-based bioinks that when combined with the appropriate chemistry, can be applied to a range of bioprinting technologies, such as extrusion or lithography-based biofabrication approaches. These bioinks are further biofunctionalised with a range of bioactive moieties, targeted for specific applications. Please see some selected publications below:

  1. H Kim; B Kang; X Cui; S-H Lee; DW Cho; TBF Woofdield; KS Lim*; J Jang*. Light-activated decellularized extracellular matrix-based bioinks for volumetric tissue analogs in centimeter-scale. Advanced Functional Materials. doi: 10.1002/adfm.202011252
  2. KS Lim*; JH Galarraga; X Cui; GCJ Lindberg; JA Burdick; TBF Woodfield. Fundamentals and applications of photocrosslinking in bioprinting. Chemical Reviews, 2020, 120 (19), 10662-10694. doi: 10.1021/acs.chemrev.9b00812
  3. KS Lim*; F Abinzano; PN Bernal; AA Sanchez; P Atienza-Roca; IA Otto; M Matsusaki; TBF Woodfield; J Malda; R Levato*. One-step crosslinking of a dual-functionalized bioink as cell carrier and cartilage-binding glue for chondral regeneration. Advanced Healthcare Materials, 2020, 9, 15, 1901792. doi: 10.1002/adhm.201901792
  4. C Alcala-Orozco; I Mutreja; X Cui; D Kumar; G Hooper; KS Lim*; TBF Woodfield*. Design and characterisation of multi-functional strontium-gelatin nanocomposite bioinks with improved print fidelity and osteogenic capacity. Bioprinting; 2020. doi: 10.1016/j.bprint.2019.e00073
  5. KS Lim; R Levato; PF Costa; MD Castilho; CR Alcala-Orozco; KV Dorenmalen; FPW Melchels; D Gawlitta; GJ Hooper; J Malda J; TBF Woodfield. Bio-resin for High Resolution Lithography-based Biofabrication of Complex Cell Laden Constructs. Biofabrication. 2018. 10 (034101). doi: 10.1088/1758-5090/aac00c
  6. KS Lim; BS Schon; NV Mekhileri; CM Chia; S Prabakar; GJ Hooper; TBF Woodfield. A New Visible Light Photo-initiating System for Improved Print Fidelity in Gelatine Based Bio-inks. ACS Biomaterials Science and Engineering, 2016. 2 (10): p. 1752–1762. doi: 10.1021/acsbiomaterials.6b00149