This online workshop is delivered by Genomics Aotearoa and NeSI, and will be taught from 10:00am-4:00pm NZT on the Thursday 15th of June, 2023. It is for New Zealand researchers interested in RNA-seq data analysis.
The focus of this workshop is to analyse RNA-seq data with the aim to identify differentially expressed genes.
Some of the topics covered in the workshop are:
– Quality assessment
– Trimming and filtering
– Mapping and read counts
– Differential expression analysis
– Over-representation analysis
Who this workshop is for
This is a beginner-friendly workshop which assumes you are familiar with the basics of R ( e.g., you can copy basic functions like head, tail, or colSums, you are aware of how R stores files as Objects) and bash (e.g., can change directory with cd command, make a new folder with mkdir command, view contents using less command). If you would like a refresher on R you can find one here. If you would like a refresher on bash you can find one here.
Setup
This is a fully online, hands-on workshop. This workshop material will be run on the NeSI High Performance Computing (HPC) platforms – there is no need to install any software for this workshop. Instructions on how to access the NeSI HPC service will be sent out with the confirmation letter to registrants.
The material for this workshop can be previewed here: https://genomicsaotearoa.github.io/RNA-seq-workshop/
Participants must have their own laptops and plan to participate actively. You will require a working web browser.
If you have any questions about these workshops, including whether they are suitable for you, please contact tyler.mcinnes@otago.ac.nz.
