{"id":149,"date":"2023-05-31T12:40:53","date_gmt":"2023-05-31T00:40:53","guid":{"rendered":"https:\/\/blogs.otago.ac.nz\/fellner\/?page_id=149"},"modified":"2025-02-17T14:57:37","modified_gmt":"2025-02-17T01:57:37","slug":"student-project-universal-stress-proteins","status":"publish","type":"page","link":"https:\/\/blogs.otago.ac.nz\/fellner\/student-project-universal-stress-proteins\/","title":{"rendered":"Universal stress proteins"},"content":{"rendered":"<p><a href=\"https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspM-crystal.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-150 size-medium\" src=\"https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspM-crystal-300x271.png\" alt=\"\" width=\"300\" height=\"271\" srcset=\"https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspM-crystal-300x271.png 300w, https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspM-crystal-332x300.png 332w, https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspM-crystal.png 639w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><a href=\"https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspY-crystal.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-151\" src=\"https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspY-crystal-300x240.png\" alt=\"\" width=\"300\" height=\"240\" srcset=\"https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspY-crystal-300x240.png 300w, https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspY-crystal-375x300.png 375w, https:\/\/blogs.otago.ac.nz\/fellner\/files\/2023\/05\/UspY-crystal.png 595w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><em>UspM and UspY crystals that can be optimised to gain high resolution data<\/em><\/p>\n<p>Supervisor: Dr. Fellner (Biochemistry) &amp; Dr. Pletzer (Microbiology and Immunology)<\/p>\n<p>The USPs projects are in collaboration with the <a href=\"https:\/\/pletzerlab.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>Pletzer Lab<\/strong> \u2013 University of Otago \u2013 Department of Microbiology and Immunology<\/a>. Co-supervision between the Fellner Biochemistry and Pletzer Microbilogy Department will strengthen these projects.<\/p>\n<p>Summary: This project is ideal if you want work on a project that links Biochemistry and Microbilogy, be exposed to both areas of research into infectious diseases. Here you will be the first in the world to determine the structure of a protein. UspM, UspN, UspK, UspX and UspY have successfully been purified in the past, showing a great potential for protein crystallisation, with crystals available for UspM and UspY but their structures have not been determined yet. A student project will focus on 1-3 USPs depending on degree level. In addition, the following techniques will investigate the oligomeric structure and potential to bind nucleotides:<\/p>\n<ul>\n<li>Isothermal titration calorimetry and Circular dichroism: Nucleotide binding preference\/strength.<\/li>\n<li>Small-angle X-ray scattering: In solution structure of oligomeric state with nucleotides.<\/li>\n<li>X-ray crystallography: Atomic information of oligomer interfaces and nucleotide binding.<\/li>\n<li>Pull-down assay combined with mass spectrometry: USPs bound by nucleotide will be immobilised on resin; exposed to <em>Pa<\/em> proteome; interactions revealed by mass spectrometry<\/li>\n<li>Bacterial growth studies: Combined with mass spectrometry metabolomics; link nucleotides to USPs during bacterial growth<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>UspM and UspY crystals that can be optimised to gain high resolution data Supervisor: Dr. Fellner (Biochemistry) &amp; Dr. Pletzer (Microbiology and Immunology) The USPs projects are in collaboration with the Pletzer Lab \u2013 University of Otago \u2013 Department of Microbiology and Immunology. Co-supervision between the Fellner Biochemistry and Pletzer Microbilogy Department will strengthen these [&hellip;]<\/p>\n","protected":false},"author":40025,"featured_media":0,"parent":0,"menu_order":14,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-149","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/pages\/149","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/users\/40025"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/comments?post=149"}],"version-history":[{"count":0,"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/pages\/149\/revisions"}],"wp:attachment":[{"href":"https:\/\/blogs.otago.ac.nz\/fellner\/wp-json\/wp\/v2\/media?parent=149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}