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Resources and Tools

Online Tools


Addgene CRISPR resources – Everything CRISPR from Addgene

BioGRID Open Repository of CRISPR Screens (ORCS) – Explanation of and links to the open respository of CRISPR Screen data

Broad institute gRNA design site

CRISPOR gRNA design tool – Online program that helps design, evaluate and clone guide sequences for the CRISPR/Cas9 system

CRISPR journal resources – Links to WEBinars, blogs and journal articles from the CRISPR journal

Horizon CRISPR learning Centre – Links to WEBinars, panel discussions and podcasts from the CRISPR journal

InDelphi Indel predication software – inDelphi is a predictive model for events following CRISPR cleavage at a specified target site that result in a non-wild type genotype detectable by Illumina sequencing. inDelphi predicts the relative frequencies of 1-bp insertion and 1- to 60-bp deletion

Off-spotter – Off-target prediction

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Additional Resources


Springer HandBook CRISPR gRNA Design 2021

Springer Handbook CRISPR-Cas Methods Vol 2 2021

Springer HandBook CRISPR-Cas Methods Vol 1 2020

Best practice for CRISPR design using current tools and resources | Elsevier Enhanced Reader

CRISPOR manual

Delivery methods

Gene Editing – Royal Society Te Apārangi Gene-Editing full documentation

Mitigating Off-target Effects

Review on Design and Analysis of CRISPR Experiments

Synthego CRISPR 101 (ebook 2019)

Thermo-Fisher Screening Resource Guide

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Journals


CRISPR Journal

CRISPR Journal Podcast

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